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abi-prism 7700 sequence detector system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher abi-prism 7700 sequence detector system
    Abi Prism 7700 Sequence Detector System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prism+7700+sequence+detector/pm39810070-75-7-7
    Average 90 stars, based on 1 article reviews
    abi-prism 7700 sequence detector system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1.
    Article Snippet: For total RNA extraction, 2x106 cells were plated for 24 h and purified using RNAeasy (Qiagen, Valencia, CA, USA). .. Two micrograms of RNA were reverse transcribed using a High Capacity cDNA Synthesis Kit (Ambion, Austin, TX, USA). mRNA expression was quantified using SYBR green chemistry and an ABI Prism 7700 Sequence Detector (Life Technologies). ..

    Article Title: Gene expression analysis of cytokines and MMPs in melatonin and rhBMP-2 enhanced bone remodeling
    Article Snippet: .. Real-time PCR (Q-PCR): The cDNA obtained in the reverse transcriptase reaction from the mRNA was amplified in Real-time PCR, in which fluorescence quantification was evaluated by the ABI Prism 7700 sequence detector (Applied Biosystems, Foster City, CA, United States). ..

    Article Title: Methods and compositions for the specific inhibition of glycolate oxidase (HAO1) by double-stranded RNA
    Article Snippet: .. PCR amplifications are performed on, for example, an ABI PRISM 7700 Sequence detector using 50 uL reactions consisting of 10 uL total RNA, 100 nM forward primer, 100 mM reverse primer, 100 nM probe, 1×TaqMan PCR reaction buffer (PE-Applied Biosystems), 5.5 mM MgCl2, 100 uM each dATP, dCTP, dGTP and dTTP, 0.2 U RNase Inhibitor (Promega), 0.025 U AmpliTaq Gold (PE-Applied Biosystems) and 0.2 U M-MLV Reverse Transcriptase (Promega). .. TAQMAN (Real-Time PCR Monitoring of Amplification) and Lightcycler Quantification of mRNA For RT-qPCR assays, total RNA is prepared from cells following DsiRNA delivery, for example, using Ambion Rnaqueous 4-PCR purification kit for large scale extractions, or Promega SV96 for 96-well assays.

    Article Title: Methods and compositions for the specific inhibition of alpha-1 antitrypsin by double-stranded RNA
    Article Snippet: .. PCR amplifications are performed on, for example, an ABI PRISM 7700 Sequence detector using 50 uL reactions consisting of 10 uL total RNA, 100 nM forward primer, 100 mM reverse primer, 100 nM probe, 1× TaqMan PCR reaction buffer (PE-Applied Biosystems), 5.5 mM MgCl2, 100 uM each dATP, dCTP, dGTP and dTTP, 0.2 U RNase Inhibitor (Promega), 0.025 U AmpliTaq Gold (PE-Applied Biosystems) and 0.2 U M-MLV Reverse Transcriptase (Promega). ..

    cDNA Synthesis:

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1.
    Article Snippet: For total RNA extraction, 2x106 cells were plated for 24 h and purified using RNAeasy (Qiagen, Valencia, CA, USA). .. Two micrograms of RNA were reverse transcribed using a High Capacity cDNA Synthesis Kit (Ambion, Austin, TX, USA). mRNA expression was quantified using SYBR green chemistry and an ABI Prism 7700 Sequence Detector (Life Technologies). ..

    Expressing:

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1.
    Article Snippet: For total RNA extraction, 2x106 cells were plated for 24 h and purified using RNAeasy (Qiagen, Valencia, CA, USA). .. Two micrograms of RNA were reverse transcribed using a High Capacity cDNA Synthesis Kit (Ambion, Austin, TX, USA). mRNA expression was quantified using SYBR green chemistry and an ABI Prism 7700 Sequence Detector (Life Technologies). ..

    SYBR Green Assay:

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1.
    Article Snippet: For total RNA extraction, 2x106 cells were plated for 24 h and purified using RNAeasy (Qiagen, Valencia, CA, USA). .. Two micrograms of RNA were reverse transcribed using a High Capacity cDNA Synthesis Kit (Ambion, Austin, TX, USA). mRNA expression was quantified using SYBR green chemistry and an ABI Prism 7700 Sequence Detector (Life Technologies). ..

    Sequencing:

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1.
    Article Snippet: For total RNA extraction, 2x106 cells were plated for 24 h and purified using RNAeasy (Qiagen, Valencia, CA, USA). .. Two micrograms of RNA were reverse transcribed using a High Capacity cDNA Synthesis Kit (Ambion, Austin, TX, USA). mRNA expression was quantified using SYBR green chemistry and an ABI Prism 7700 Sequence Detector (Life Technologies). ..

    Article Title: Methods for labeling DNA fragments to reconstruct physical linkage and phase
    Article Snippet: .. In other cases, a qPCR reaction uses fluorescent Taqman methodology and an instrument capable of measuring fluorescence in real time (e.g., ABI Prism 7700 Sequence Detector). ..

    Article Title: Gene expression analysis of cytokines and MMPs in melatonin and rhBMP-2 enhanced bone remodeling
    Article Snippet: .. Real-time PCR (Q-PCR): The cDNA obtained in the reverse transcriptase reaction from the mRNA was amplified in Real-time PCR, in which fluorescence quantification was evaluated by the ABI Prism 7700 sequence detector (Applied Biosystems, Foster City, CA, United States). ..

    Article Title: Methods and compositions for the specific inhibition of glycolate oxidase (HAO1) by double-stranded RNA
    Article Snippet: .. PCR amplifications are performed on, for example, an ABI PRISM 7700 Sequence detector using 50 uL reactions consisting of 10 uL total RNA, 100 nM forward primer, 100 mM reverse primer, 100 nM probe, 1×TaqMan PCR reaction buffer (PE-Applied Biosystems), 5.5 mM MgCl2, 100 uM each dATP, dCTP, dGTP and dTTP, 0.2 U RNase Inhibitor (Promega), 0.025 U AmpliTaq Gold (PE-Applied Biosystems) and 0.2 U M-MLV Reverse Transcriptase (Promega). .. TAQMAN (Real-Time PCR Monitoring of Amplification) and Lightcycler Quantification of mRNA For RT-qPCR assays, total RNA is prepared from cells following DsiRNA delivery, for example, using Ambion Rnaqueous 4-PCR purification kit for large scale extractions, or Promega SV96 for 96-well assays.

    Article Title: Polyetheretherketone Double Functionalization with Bioactive Peptides Improves Human Osteoblast Response
    Article Snippet: For cDNA synthesis, 5 μg of total RNA were reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Applied Biosystems, Milan, Italy) with random primers. .. Quantitative polymerase chain reaction (qPCR) was performed on an ABI Prism 7700 sequence detector (Applied Biosystems) for 40 cycles at an annealing temperature of 60 °C. .. The reactions were carried out with TaqMan Universal PCR Master Mix and Universal Probe Library (Roche, Milan, Italy), which included a fluorescent dye, as per the manufacturer’s instructions.

    Article Title: Enhanced and cross-reactive in vitro memory B cell response against Epstein-Barr virus nuclear antigen 1 in multiple sclerosis
    Article Snippet: Quantification of EBV DNA copies was performed by TaqMan real-time PCR technique (Applied Biosystems, Massachusetts, USA) as described previously ( ) with modified primers for the conserved BamH1 W fragment of EBV (5′-CTTCTCAGTCCAGCGCGTTT-3′ and 5′-CAGTGGTCCCCCTCCCTAGA-3′) and a fluorogenic probe (5′-FAM CGTAAGCCAGACAGCAGCCAATTGTCAG-TAMRA-3′). .. The PCR was run on an ABI Prism 7700 Sequence Detector (Applied Biosystems, Massachusetts, USA), and samples were analyzed in duplicates. ..

    Article Title: Methods and compositions for the specific inhibition of alpha-1 antitrypsin by double-stranded RNA
    Article Snippet: .. PCR amplifications are performed on, for example, an ABI PRISM 7700 Sequence detector using 50 uL reactions consisting of 10 uL total RNA, 100 nM forward primer, 100 mM reverse primer, 100 nM probe, 1× TaqMan PCR reaction buffer (PE-Applied Biosystems), 5.5 mM MgCl2, 100 uM each dATP, dCTP, dGTP and dTTP, 0.2 U RNase Inhibitor (Promega), 0.025 U AmpliTaq Gold (PE-Applied Biosystems) and 0.2 U M-MLV Reverse Transcriptase (Promega). ..

    Article Title: Unravelling the Programmed Inflammation and Tissue Repair by a Multipotential Antimicrobial K21 Silane.
    Article Snippet: The SYBR Green PCR Kit from Applied Biosystems in Carlsbad, CA was utilised for quantitative realtime RT-PCR analysis. .. The measurement of fluorescence in real-time was performed using an ABI PRISM 7700 Sequence Detector manufactured by Applied Biosystems. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Methods for labeling DNA fragments to reconstruct physical linkage and phase
    Article Snippet: .. In other cases, a qPCR reaction uses fluorescent Taqman methodology and an instrument capable of measuring fluorescence in real time (e.g., ABI Prism 7700 Sequence Detector). ..

    Article Title: Gene expression analysis of cytokines and MMPs in melatonin and rhBMP-2 enhanced bone remodeling
    Article Snippet: .. Real-time PCR (Q-PCR): The cDNA obtained in the reverse transcriptase reaction from the mRNA was amplified in Real-time PCR, in which fluorescence quantification was evaluated by the ABI Prism 7700 sequence detector (Applied Biosystems, Foster City, CA, United States). ..

    Article Title: Polyetheretherketone Double Functionalization with Bioactive Peptides Improves Human Osteoblast Response
    Article Snippet: For cDNA synthesis, 5 μg of total RNA were reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Applied Biosystems, Milan, Italy) with random primers. .. Quantitative polymerase chain reaction (qPCR) was performed on an ABI Prism 7700 sequence detector (Applied Biosystems) for 40 cycles at an annealing temperature of 60 °C. .. The reactions were carried out with TaqMan Universal PCR Master Mix and Universal Probe Library (Roche, Milan, Italy), which included a fluorescent dye, as per the manufacturer’s instructions.

    Fluorescence:

    Article Title: Methods for labeling DNA fragments to reconstruct physical linkage and phase
    Article Snippet: .. In other cases, a qPCR reaction uses fluorescent Taqman methodology and an instrument capable of measuring fluorescence in real time (e.g., ABI Prism 7700 Sequence Detector). ..

    Article Title: Gene expression analysis of cytokines and MMPs in melatonin and rhBMP-2 enhanced bone remodeling
    Article Snippet: .. Real-time PCR (Q-PCR): The cDNA obtained in the reverse transcriptase reaction from the mRNA was amplified in Real-time PCR, in which fluorescence quantification was evaluated by the ABI Prism 7700 sequence detector (Applied Biosystems, Foster City, CA, United States). ..

    Article Title: Unravelling the Programmed Inflammation and Tissue Repair by a Multipotential Antimicrobial K21 Silane.
    Article Snippet: The SYBR Green PCR Kit from Applied Biosystems in Carlsbad, CA was utilised for quantitative realtime RT-PCR analysis. .. The measurement of fluorescence in real-time was performed using an ABI PRISM 7700 Sequence Detector manufactured by Applied Biosystems. ..

    Amplification:

    Article Title: Gene expression analysis of cytokines and MMPs in melatonin and rhBMP-2 enhanced bone remodeling
    Article Snippet: .. Real-time PCR (Q-PCR): The cDNA obtained in the reverse transcriptase reaction from the mRNA was amplified in Real-time PCR, in which fluorescence quantification was evaluated by the ABI Prism 7700 sequence detector (Applied Biosystems, Foster City, CA, United States). ..

    Polymerase Chain Reaction:

    Article Title: Methods and compositions for the specific inhibition of glycolate oxidase (HAO1) by double-stranded RNA
    Article Snippet: .. PCR amplifications are performed on, for example, an ABI PRISM 7700 Sequence detector using 50 uL reactions consisting of 10 uL total RNA, 100 nM forward primer, 100 mM reverse primer, 100 nM probe, 1×TaqMan PCR reaction buffer (PE-Applied Biosystems), 5.5 mM MgCl2, 100 uM each dATP, dCTP, dGTP and dTTP, 0.2 U RNase Inhibitor (Promega), 0.025 U AmpliTaq Gold (PE-Applied Biosystems) and 0.2 U M-MLV Reverse Transcriptase (Promega). .. TAQMAN (Real-Time PCR Monitoring of Amplification) and Lightcycler Quantification of mRNA For RT-qPCR assays, total RNA is prepared from cells following DsiRNA delivery, for example, using Ambion Rnaqueous 4-PCR purification kit for large scale extractions, or Promega SV96 for 96-well assays.

    Article Title: Enhanced and cross-reactive in vitro memory B cell response against Epstein-Barr virus nuclear antigen 1 in multiple sclerosis
    Article Snippet: Quantification of EBV DNA copies was performed by TaqMan real-time PCR technique (Applied Biosystems, Massachusetts, USA) as described previously ( ) with modified primers for the conserved BamH1 W fragment of EBV (5′-CTTCTCAGTCCAGCGCGTTT-3′ and 5′-CAGTGGTCCCCCTCCCTAGA-3′) and a fluorogenic probe (5′-FAM CGTAAGCCAGACAGCAGCCAATTGTCAG-TAMRA-3′). .. The PCR was run on an ABI Prism 7700 Sequence Detector (Applied Biosystems, Massachusetts, USA), and samples were analyzed in duplicates. ..

    Article Title: Methods and compositions for the specific inhibition of alpha-1 antitrypsin by double-stranded RNA
    Article Snippet: .. PCR amplifications are performed on, for example, an ABI PRISM 7700 Sequence detector using 50 uL reactions consisting of 10 uL total RNA, 100 nM forward primer, 100 mM reverse primer, 100 nM probe, 1× TaqMan PCR reaction buffer (PE-Applied Biosystems), 5.5 mM MgCl2, 100 uM each dATP, dCTP, dGTP and dTTP, 0.2 U RNase Inhibitor (Promega), 0.025 U AmpliTaq Gold (PE-Applied Biosystems) and 0.2 U M-MLV Reverse Transcriptase (Promega). ..



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